primary antibodies against ace2 nbpi-76614 Search Results


primary antibodies against ace2  (Novus Biologicals)
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Novus Biologicals primary antibodies against ace2
The effect of ischemia reperfusion injury (IRI) on renal angiotensin-converting enzyme 2 <t>(ACE2),</t> heme oxygenase-1 (HO-1), and HO-2 expression in mice 1 day after 22.5 minutes of ischemia. (A) ACE2 (left panel), HO-1 (middle panel), and HO-2 (right panel) mRNA expression in mice subjected to sham or IRI as measured by quantitative real-time RT-PCR. (B) Western blot analysis of ACE2, HO-1, and HO-2 protein expression with assessment of equal loading by immunoblotting for GAPDH. (C) Normalized densitometric analysis of Western blots of ACE2 (left panel), HO-1 (middle panel), and HO-2 (right panel) expression. n = 4 and n = 5 in sham and IRI groups, respectively; *P<0.05 versus sham group.
Primary Antibodies Against Ace2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of ischemia reperfusion injury (IRI) on renal angiotensin-converting enzyme 2 (ACE2), heme oxygenase-1 (HO-1), and HO-2 expression in mice 1 day after 22.5 minutes of ischemia. (A) ACE2 (left panel), HO-1 (middle panel), and HO-2 (right panel) mRNA expression in mice subjected to sham or IRI as measured by quantitative real-time RT-PCR. (B) Western blot analysis of ACE2, HO-1, and HO-2 protein expression with assessment of equal loading by immunoblotting for GAPDH. (C) Normalized densitometric analysis of Western blots of ACE2 (left panel), HO-1 (middle panel), and HO-2 (right panel) expression. n = 4 and n = 5 in sham and IRI groups, respectively; *P<0.05 versus sham group.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: The effect of ischemia reperfusion injury (IRI) on renal angiotensin-converting enzyme 2 (ACE2), heme oxygenase-1 (HO-1), and HO-2 expression in mice 1 day after 22.5 minutes of ischemia. (A) ACE2 (left panel), HO-1 (middle panel), and HO-2 (right panel) mRNA expression in mice subjected to sham or IRI as measured by quantitative real-time RT-PCR. (B) Western blot analysis of ACE2, HO-1, and HO-2 protein expression with assessment of equal loading by immunoblotting for GAPDH. (C) Normalized densitometric analysis of Western blots of ACE2 (left panel), HO-1 (middle panel), and HO-2 (right panel) expression. n = 4 and n = 5 in sham and IRI groups, respectively; *P<0.05 versus sham group.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Angiotensin-converting enzyme 2 and heme oxygenase-1 expression in the murine ischemia reperfusion injury model at 2 days post ischemia

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: Angiotensin-converting enzyme 2 and heme oxygenase-1 expression in the murine ischemia reperfusion injury model at 2 days post ischemia

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Activity Assay

Localization of renal ACE2 and NGAL protein expression in the kidney 2 days after IRI (18 minutes). Immunofluorescence staining for DAPI (blue), ACE2 (red), and NGAL (green) in kidneys of mice two days after sham (A) or IRI (B). There is reduced tubular ACE2 staining in kidneys of mice subjected to IRI accompanied by increased NGAL staining in tubules. High power insets of (A) and (B) are shown in (C) and (D) respectively. After IRI, apical ACE2 expression markedly decreased especially in the proximal tubules. Scale bar = 50 µm.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: Localization of renal ACE2 and NGAL protein expression in the kidney 2 days after IRI (18 minutes). Immunofluorescence staining for DAPI (blue), ACE2 (red), and NGAL (green) in kidneys of mice two days after sham (A) or IRI (B). There is reduced tubular ACE2 staining in kidneys of mice subjected to IRI accompanied by increased NGAL staining in tubules. High power insets of (A) and (B) are shown in (C) and (D) respectively. After IRI, apical ACE2 expression markedly decreased especially in the proximal tubules. Scale bar = 50 µm.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Immunofluorescence, Staining

Renal HO-1 and ACE2 mRNA expression in mice after LPS administration. HO-1 and ACE2 mRNA expression was assessed in the kidneys of mice at 4, 8, and 15 hours after saline or LPS (10 mg/kg, intravenous, IV) treatment. mRNA expression was determined by quantitative real-time RT-PCR. n = 5 and n = 5–6 in saline-treated and LPS-treated groups, respectively; *P<0.05 vs saline-treated group for that timepoint.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: Renal HO-1 and ACE2 mRNA expression in mice after LPS administration. HO-1 and ACE2 mRNA expression was assessed in the kidneys of mice at 4, 8, and 15 hours after saline or LPS (10 mg/kg, intravenous, IV) treatment. mRNA expression was determined by quantitative real-time RT-PCR. n = 5 and n = 5–6 in saline-treated and LPS-treated groups, respectively; *P<0.05 vs saline-treated group for that timepoint.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Saline, Quantitative RT-PCR

Localization of renal ACE2 protein expression in the kidney 15 hours after LPS administration. Immunofluorescence staining for DAPI (blue) and ACE2 (red) in kidneys of mice at 15 hours after saline (A) or LPS (10 mg/kg, IV) administration (B). After LPS administration, increased ACE2 expression in tubules of the murine kidney was observed. High power insets of (A) and (B) showing the localization of ACE2 expression in proximal tubules is shown in (C) and (D) respectively. Scale bar = 50 µm.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: Localization of renal ACE2 protein expression in the kidney 15 hours after LPS administration. Immunofluorescence staining for DAPI (blue) and ACE2 (red) in kidneys of mice at 15 hours after saline (A) or LPS (10 mg/kg, IV) administration (B). After LPS administration, increased ACE2 expression in tubules of the murine kidney was observed. High power insets of (A) and (B) showing the localization of ACE2 expression in proximal tubules is shown in (C) and (D) respectively. Scale bar = 50 µm.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Immunofluorescence, Staining, Saline

Renal ACE2 mRNA expression in HO-1+/+ and HO-1−/− mice after LPS administration. ACE2 mRNA expression was assessed in the kidneys of HO-1+/+ and HO-1−/− mice 1 day after saline or LPS (2 mg/kg, intraperitoneal, IP) treatment. mRNA expression was assessed by quantitative real-time RT-PCR. n = 6 in each group. *P<0.05 versus saline-treated HO-1+/+ group.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: Renal ACE2 mRNA expression in HO-1+/+ and HO-1−/− mice after LPS administration. ACE2 mRNA expression was assessed in the kidneys of HO-1+/+ and HO-1−/− mice 1 day after saline or LPS (2 mg/kg, intraperitoneal, IP) treatment. mRNA expression was assessed by quantitative real-time RT-PCR. n = 6 in each group. *P<0.05 versus saline-treated HO-1+/+ group.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Saline, Quantitative RT-PCR

The effect of sex on renal ACE2 and HO-1 protein expression in mice. Western blot analysis of renal ACE2 and HO-1 protein expression was determined in male and female mice with assessment of equal loading by immunoblotting for GAPDH. Normalized densitometric analysis for ACE2 (left panel) and HO-1 (right panel) is displayed below the Western blots. n = 8 in each group; *P<0.05 versus male group.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: The effect of sex on renal ACE2 and HO-1 protein expression in mice. Western blot analysis of renal ACE2 and HO-1 protein expression was determined in male and female mice with assessment of equal loading by immunoblotting for GAPDH. Normalized densitometric analysis for ACE2 (left panel) and HO-1 (right panel) is displayed below the Western blots. n = 8 in each group; *P<0.05 versus male group.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Western Blot

The effect of age on renal ACE2 and HO-1 protein expression in mice. Western blot analysis of renal ACE2 and HO-1 protein expression was determined in young (10 weeks old) and old (22–23 months old) male mice with assessment of equal loading by immunoblotting for GAPDH. Normalized densitometric analysis for ACE2 (left panel) and HO-1 (right panel) is displayed below the Western blots. n = 8 and n = 11 in young and old groups, respectively; *P<0.05.

Journal: Kidney360

Article Title: Expression of ACE2 in the Intact and Acutely Injured Kidney

doi: 10.34067/KID.0001562021

Figure Lengend Snippet: The effect of age on renal ACE2 and HO-1 protein expression in mice. Western blot analysis of renal ACE2 and HO-1 protein expression was determined in young (10 weeks old) and old (22–23 months old) male mice with assessment of equal loading by immunoblotting for GAPDH. Normalized densitometric analysis for ACE2 (left panel) and HO-1 (right panel) is displayed below the Western blots. n = 8 and n = 11 in young and old groups, respectively; *P<0.05.

Article Snippet: Primary antibodies against ACE2 (catalog NBPI-76614, Novus Biologicals) and NGAL (catalog AF1757, R&D Systems, Minneapolis, MN) were used.

Techniques: Expressing, Western Blot